SLEEP DEPRIVATION SYSTEM
Pinnacle's AUTOMATED SLEEP DEPRIVATION SYSTEM offers a unique solution for sleep deprivation and sleep fragmentation studies. It provides user controls for sleep depriving mice
and rats without direct human intervention while minimizing exercise. The system is sold as a CORE SYSTEM and Pinnacle's FEEDBACK PRO SOFTWARE and EEG HARDWARE can be added for additional
functionality. By adding Feedback EEG/EMG signals can be used to determine sleep/wake state and initiate deprivation as required.
Tutorial Tip: Secure crossbar with teflon tape.
EFFECTIVENESS OF SLEEP DEPRIVATION IN MICE
The chart to the right shows a comparison of baseline sleep (purple bars) with a 24-hour sleep deprivation period (gold bars). Six mice (male, C57BL/6J) were maintained on a 12-hour lights-on (horizontal tan)/ 12-hour lights-off (horizontal black) schedule. EEG and EMG activity were recorded for 24 hours (baseline). During the following 24 hours, each animal was sleep deprived by programming the system to rotate the bar within ten seconds of the mouse entering a sleep-like state, as determined by active EEG feedback. Vertical bars represent sleep time in 2-hour bins.